Abstract：Objective ： To investigate the effect and mechanism of microtubule stabilizer Epothilone D on the
excitatory synaptic structure of cortical neurons in BTBR mouse with autism spectrum disorder. Methods ： The
primary cerebral cortical neurons of BTBR mouse were isolated and cultured， and the purity of cultured neurons
was detected by immunofluorescence technology. Cortical neurons of relatively mature were randomly divided into
an experimental group （epothilone D group） and a vehicle control group （0.1% v/v DMSO） for 24 h intervention.
After cold treatment for 90 min， the morphological changes of microtubules were observed by microtubule
immunofluorescence staining. Western blotting was used to detect the expression of acetyl-tubulin， α-tubulin，
MAP2， STOP， VGLUT1， PSD95， GluN2B and mGluR5 proteins. Results ：Compared with the control group，
the cold stability of microtubules in cortical neuron of BTBR mouse was increased with 10 nmol/L Epothilone D.
The expressions of excitatory synapse-related and excitatory glutamate receptor-related proteins of cortical neurons
in BTBR mouse were increased. Conclusion ： The microtubule stabilizer epothilone D can improve the excitatory
synaptic structure of cortical neurons in BTBR mouse in vitro， which may be related to the increase of microtubule
stability.mouse
