目的：探讨p53 和DNA损伤调节基因1（PDRG1）在口腔癌组织中的表达及通过Wnt/β-catenin 信号途径对 口腔癌细胞自噬和凋亡的影响。方法：收集2017 年6 月至2019 年6 月在河南省第二人民医院60 例接受口腔癌切 除手术的患者的癌组织标本及配对的癌旁组织，qRT-PCR 和免疫印迹检测PDRG1 在口腔癌组织中的mRNA和蛋 白表达；同时体外培养正常人口腔角质形成细胞（NHOK）和口腔癌细胞系，qRT-PCR 和免疫印迹检测PDRG1 在口腔癌细胞系中的mRNA和蛋白表达；将口腔癌细胞系SCC-15 细胞分为shNC 组和shPDRG1 组，细胞集落形 成实验检测细胞增殖能力；免疫印迹检测细胞中LC3Ⅱ/LC3Ⅰ蛋白表达比例；透射电镜检测细胞中自噬体数量； 流式细胞术检测细胞凋亡率；免疫印迹检测Wnt/β-catenin 信号途径相关蛋白表达。结果：PDRG1 在口腔癌组织 和口腔癌细胞系中高表达；与shNC 组相比，shPDRG1 组SCC-15 细胞中PDRG1 mRNA 的表达显著下降， PDRG1 蛋白的表达水平明显下调。与shNC 组相比，shPDRG1 组SCC-15 细胞的集落数目明显减少，细胞中LC3Ⅱ/LC3Ⅰ 的比例减少；与shNC 组比较，shPDRG1 组细胞中自噬体数量减少，细胞的凋亡率明显提高，细胞中β-catenin、 c-Myc、cyclin D1 蛋白表达水平下调。结论：PDRG1 在口腔癌组织和口腔癌细胞系中高表达，沉默PDRG1 抑制 SCC-15 细胞增殖和自噬，增强SCC-15 细胞的凋亡率及抑制Wnt/β-catenin 信号途径。

Objective ： To investigate the expression of p53 and DNA damage regulated 1（ PDRG1） in oral cancer tissues and its effect through Wnt/β-catenin signaling pathway on autophagy and apoptosis of oral cancer cells. Methods ： Cancer tissue specimens and matched paracancerous tissues were collected from 60 patients undergoing oral cancer resection. qRT-PCR and Western blotting were used to detect the mRNA and protein expression of PDRG1 in oral cancer tissues. At the same time， normal oral keratinocytes（ NHOK）and oral cancer cell lines were cultured in vitro. qRT-PCR and Western blotting were used to detect the mRNA and protein expression of PDRG1 in oral cancer cell lines. SCC-15 cells were divided into shNC group and shPDRG1 group. Cell colony formation experiment was used to detect cell proliferation ability. The ratio of LC3Ⅱ/LC3Ⅰ protein expression in cells was detected by Western blotting. Electron microscopy was used to detect the number of autophagosomes in cells. The rate of cell apoptosis was detected by cell flow cytometry. Western blotting was used to detect the expression of proteins related to the Wnt/β-catenin signaling pathway. Results ： PDRG1 was highly expressed in oral cancer tissues and oral cancer cell lines. Compared with the shNC group， the expression of PDRG1 mRNA and protein in SCC-15 cells in the shPDRG1 group was significantly decreased， the number of colonies of SCC-15 cells in the shPDRG1 group was significantly reduced， and the ratio of LC3Ⅱ/LC3Ⅰ in the shPDRG1 group was reduced. Compared with the shNC group， the number of autophagosomes in the shPDRG1 group was decreased， the apoptosis rate of SCC-15 cells in the shPDRG1 group was significantly increased， and the protein expression levels of β-catenin， c-myc， and cyclin D1 in the SCC- 15 cells in the shPDRG1 group were down-regulated. Conclusion ： PDRG1 is highly expressed in oral cancer tissues and oral cancer cell lines. Silencing PDRG1 inhibits the proliferation and autophagy of SCC-15 cells， enhances the apoptosis rate of SCC-15 cells ， and inhibits the Wnt/β-catenin signaling pathway.