Abstract：Objective ： To explore the effect of interferon regulatory factor 3 （IRF3） on the expression of
interleukin-17 （IL-17） in mouse peritoneal macrophages stimulated by lipopolysaccharide（ LPS）. Methods ： Mice
were intraperitoneally injected with 3% thioglycolic acid broth. Three days later， the peritoneal macrophages of
C57BL/6 wild and IRF3 gene knockout mice were extracted and cultured overnight， then stimulated with LPS.
Enzyme linked immunosorbent assay was used to detect the expression of cytokines IL-17 and interleukin-6（ IL-6）
in cell culture supernatant. Western blotting was used to detect the expression of inhibitor of nuclear factor kappa
B （IκB） α， IRF3， phosphorylated signal transducer and transcription activator 3 （STAT 3） in cells. Results ： After
LPS stimulation， the expression of IL-17 in mouse peritoneal macrophages was significantly promoted by IRF3，
accompanied by the production of IL-6， the degradation of IκBα， and STAT3 phosphorylation. Conclusions ：
IRF3 may indirectly activate STAT3 phosphorylation by promoting the production of IL-6， thereby promoting the
production of IL-17.