Abstract：Objective ： To investigate the effect of miR-30c on learning and memory of APP/PS1 transgenic mice
through regulating the hippocampal neurogenesis. Methods ： The immunofluorescence staining of Ki67 cells was
detected to investigatethe hippocampal neurogenesis, and Morris maze was adopted to detect the learning and
memory of APP/PS1 transgenic mice. With the method of stereological localization and miR-30c over-expression
and miR-30c knockdown lentiviruses were microinjected into the dentate gyrus of hippocampi to modulate the
neurogenesis. Results ：The hippocampal neurogenesis, and learning and memory capacity of APP/PS1 transgenic
mice were strikingly lower than that of the wild type mice. However, when miR-30c was overexpressed in the
hippocampi of APP/PS1 transgenic mice, the hippocampal neurogenesis was evidently increased（ 14.2 fold）；
In contrast, when miR-30c was knocked down in the hippocampi of APP/PS1 transgenic mice, the hippocampal
neurogenesis was prominently decreased （0.25 fold）. Moreover, results of Morris maze demonstrated that when
miR-30c in hippocampi of APP/PS1 transgenic mice was knocked down the learning and memory capacity was
significantly declined, when miR-30c in hippocampi of APP/PS1 transgenic mice was overexpressed, the learning and
memory capacity was obvious increased. Conclusion ： miR-30c probably influence learning and memory through
regulating hippocampal neurogenesis, indicating that miR-30c might be a potential therapeutic target for modulating
neurogenesis, and learning and memory capacity of Alzheimer's diseases.
